Treatment with both autophagy modulators caused changes to autophagy dye staining of cells. The assay platform described in this tutorial offers unique advantages for high-throughput screening: it ...

Live-cell imaging for hypoxia-induced autophagy assay. The microincubator controller was connected to a premixed gas tank containing 95% air and 5% CO 2 (normoxic gas line) and purged.

Autophagy is the major intracellular degradation route in mammalian cells. ... Klionsky DJ. The quantitative Pho8Delta60 assay of nonspecific autophagy. Methods Enzymol 2008;451:33-42. Crossref.

An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure. Nature Communications , 2018; 9 (1) DOI: 10.1038/s41467-018-05254-w Cite This Page : ...

Autophagy is the body’s way of cleaning out damaged cells, in order to regenerate newer, healthier cells. It allows your body to remove the dysfunctional cells.

Autophagy (self eating) ... So the accumulation of free GFP serves as a surrogate for autophagy. It is this assay that allowed Marshall to quickly find the Cue5 receptor.

Autophagy is a natural process in which the body’s cells clean out any damaged or unnecessary components. Learn more about its effects in this article.

Autophagy also affects the levels of specific hormones, including glucagon, insulin, and ghrelin, thus decreasing your hunger. These effects could promote weight loss by reducing the number of ...

The mechanics behind how an important process within the cell traps material before recycling it has puzzled scientists for years. But Penn State researchers have gained new insight into how this ...