Gold standard assay techniques include the enzyme-linked immunosorbent assay (ELISA), western blotting, and radioimmunoassay techniques. Antibody assays are widely used in clinical diagnostics and ...

From ELISA to western blotting, getting reliable experimental results needs good technique. With some systematic thinking, plus a bit of trial and error, you can improve your antibody-handling skills.

Western blotting allows scientists to identify specific proteins in complex biological samples and determine relative abundance of a protein target. Login. ... García-Cisneros S, et al. Performance of ...