To develop a kinase-substrate map for eukaryotes, we determined the substrates recognized by 87 different yeast protein kinases and bovine protein kinase A, by using a yeast proteome array and the ...

Protein kinase ... a PrKX substrate, and PrKX, which undergoes autophosphorylation, might result in the identification of a conserved PrKX phosphorylation target. Indeed, this alignment resulted ...

In a phenomenon called phospho-priming, phosphorylation of a protein by one kinase “primes” the substrate by creating a knock-on motif that can be recognized by a second kinase: the first ...

Phosphorylation is arguably the most ... colorimetric detection of captured protein using TMB substrate solution. With minimal hands-on time, this assay format removes the burden of running ...

Dysregulation of protein phosphorylation plays a major role ... has established that all eukaryotic kinases share a common set of substrates, nine amino acid segments shared by all proteins ...

Understanding neural protein phosphorylation and the role of different kinases and substrates is now made easier by the newly developed data repository, KANPHOS Signal transduction, or cellular ...

MARCKS, the myristoylated alanine-rich C kinase substrate, has been studied in non-neuronal cells for years as a regulator of cell shape and motility. A few researchers have noted alterations in ...

1) Protein phosphorylation After proteins are produced ... phosphate groups to proteins using adenosine triphosphate as a substrate are called protein kinases, and enzymes that catalyze the ...

These include the use of protein microarrays, peptide libraries, analog-sensitive kinase alleles and polyacrylamide gels in which the kinase or substrate of interest has been polymerized.

In the September 20 Neuron, researchers led by Bart De Strooper and Patrik Verstreken of K.U. Leuven, Belgium, identify EndophilinA (EndoA), a protein important for synaptic vesicle endocytosis, as a ...

Ro-31-8425, a potent and selective inhibitor of protein kinase C, showed inhibition of phosphorylation for many of the substrate peptides, including substrate peptides for RSK (ribosomal s6 kinase ...

[26–28] Such approaches also led to the identification of 65 candidate substrates ... these phosphorylation studies by using this same approach to analyze nearly every yeast protein kinase ...