My cry of frustration echoed down the hall. My latest attempt to subclone five important PCR products had failed yet again. After two months of fruitless effort, I was at my wit's end until a ...

All cloning was carried out using a TOPO TA Cloning kit (Invitrogen, UK) according to the manufacturers instructions. TOPO TA cloning takes advantage of the nontemplate-dependent terminal ...

We amplified the adult zebrafish ttn N2B exon using the Expand 20 kbPlus PCR System (Roche), cloned the 4.6-kb PCR product using a TOPO TA Cloning Kit (Invitrogen) and determined the sequence ...

it also can provide PCR amplicons suitable for use with TOPO TA Cloning® or pGEM® T-Easy cloning vectors. For cloning small RNAs lacking the 5’-phosphorylated end present in miRNAs, a modified kit - ...