To develop a kinase-substrate map for eukaryotes, we determined the substrates recognized by 87 different yeast protein kinases and bovine protein kinase A, by using a yeast proteome array and the ...

Protein kinase ... a PrKX substrate, and PrKX, which undergoes autophosphorylation, might result in the identification of a conserved PrKX phosphorylation target. Indeed, this alignment resulted ...

[26–28] Such approaches also led to the identification of 65 candidate substrates ... these phosphorylation studies by using this same approach to analyze nearly every yeast protein kinase ...

Understanding neural protein phosphorylation and the role of different kinases and substrates is now made easier by the newly developed data repository, KANPHOS Signal transduction, or cellular ...

Dysregulation of protein phosphorylation plays a major role ... has established that all eukaryotic kinases share a common set of substrates, nine amino acid segments shared by all proteins ...

Ro-31-8425, a potent and selective inhibitor of protein kinase C, showed inhibition of phosphorylation for many of the substrate peptides, including substrate peptides for RSK (ribosomal s6 kinase ...

Phosphorylation can have profound effects on protein conformation and function that regulate many cellular processes, including the complex cascade of events that control cell cycle progression. The ...

1) Protein phosphorylation After proteins are produced ... phosphate groups to proteins using adenosine triphosphate as a substrate are called protein kinases, and enzymes that catalyze the ...

Protein phosphorylation is one of the most important post-translational ... to show protein bands by autoradiography or substrate color development. After passing through the concentrated and ...

In the September 20 Neuron, researchers led by Bart De Strooper and Patrik Verstreken of K.U. Leuven, Belgium, identify EndophilinA (EndoA), a protein important for synaptic vesicle endocytosis, as a ...

scientists explore how a brain protein called tau changes its behavior when a small chemical group called a phosphate attaches to it—a process known as phosphorylation. Under normal conditions ...

These include the use of protein microarrays, peptide libraries, analog-sensitive kinase alleles and polyacrylamide gels in which the kinase or substrate of interest has been polymerized.