Pluripotent stem cells (PSCs) have been invaluable tools ... Another significant advancement was the establishment of a feeder-free culture system for naive PSCs. Traditional methods for culturing ...

However, developing and maintaining the naïve state of primate pluripotent stem cells (PSCs) remains difficult as does in vivo detection of PSCs, thus limiting biotechnology application in the ...

The reprogrammed cells are known as induced pluripotent stem cells (iPSCs). In culture, pluripotency must be maintained when iPSCs undergo proliferation in advance of differentiation. Production ...

Pluripotent stem cells (PSCs) include embryonic stem cells ... through the overexpression of four transcription factors in culture, rendering them immortal. Patient-specific human iPSCs can ...

As stem cell cultures are very sensitive and respond to the smallest influence, stable culture conditions are crucial to maintain reliability and reproducibility of experimental data. In this ...

both pluripotent embryonic stem cells and induced pluripotent stem cells (iPSCs) need to be expanded by numerous passages in culture in vitro because they begin with very small colonies of cells ...

In other words, a culture with a million healthy cells ... target for repair with regenerative medicine using human pluripotent stem cells,” noted Dr. Eggan. Those organs include the pancreas ...

Here we describe a straightforward culture protocol for the expansion of undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) in suspension culture.

Soft substrate promotes pluripotent stem cell culture Date: December 19, 2010 Source: University of Illinois at Urbana-Champaign Summary: Researchers have found a key to keeping stem cells in ...

University of Illinois researchers have found a key to keeping stem cells in their neutral state: It takes a soft touch. "This has huge applications in the future of regenerative medicine," said ...

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