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RNA Isolation. Total RNA was isolated from cultures with TRI reagent (Molecular Research Center), following the manufacturer’s instructions. The concentration was measured using a NanoDrop ND-1000 ...
Total RNA was isolated from 0.75 million HeLa cells using Norgen's Total RNA Purification Kit, various silica-based competitors and phenol-based TRI Reagent RT-PCR was performed according to Shi ...
For this, the cells were transfected [by either Lipofectamine (endosomal) or Nucleofection (nonendosomal)] in a 12-well plate and incubated with the nanoluciferase mRNA for 3, 6, and 24 h. The cells ...
The Global DNA and RNA Extraction Market is projected to reach USD 7.9 billion in 2024 and is expected to grow to USD 16.4 billion by 2033, with a CAGR of 8.4%. The global DNA & RNA extraction ...
9) For RNA extraction, add Trizol (TRI Reagent- Zymo Research) directly on the washed beads and vortex thoroughly for 2 min to detach the probes. Place back the samples on the magnetic rack and ...
RNA Extraction Service. Exosomes are small endosome-derived lipid nanoparticles actively secreted by exocytosis in most living cells. Exosomes have shown pleiotropic physiological and pathological ...
Cells were homogenized in TRI‐reagent and RNA was extracted using one of three different methods. (i) RNA extraction was performed following the manufacturer’s protocol, and then the extracted RNA was ...
RNA isolation and enrichment reagent are used to isolate RNA from human, animal, plant, yeast, bacteria or virus and can perform well in large tissue by simultaneous extraction. RNA extracted with the ...