Catalase test: Principle, Procedure, Results, Uses • Microbe Online
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Catalase test: Principle, Procedure, Results, Uses • Microbe Online
Oct 7, 2013
microbeonline.com
The catalase test is a quick and easy method for the differentiation of Streptococcus and Staphylococcus. All Streptococcus species are catalase negative and Staphylococcus are catalase positive (bubble production) The medium used should NOT have blood incorporated since erythrocytes have catalase activity, which can give false positive results. The test should be done on young cultures 18-24 hours. The reagent used is 3% hydrogen peroxide | Medical Lab Technologist
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The catalase test is a simple and fundamental biochemical assay used in microbiology to differentiate between types of microorganisms, specifically bacteria. It determines whether a microorganism produces the enzyme catalase. ​🧪 The Science Behind It ​The purpose of the catalase enzyme is to protect the cell from toxic byproducts of aerobic respiration. When a cell uses oxygen, it inevitably produces reactive oxygen species (ROS), a common one being hydrogen peroxide (H_2O_2). Hydrogen peroxide
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